Archive for the ‘Biology’ Category

Is there a different type of primer i have to use when running PCR with genomic DNA compared to cDNA?

Sunday, February 1st, 2009

bob oso asked: Im still in school but when i run a PCR, I normally isolate RNA first then RT to cDNA and run PCR with that. This time i isolated the genomic DNA from the sample and ran PCR using the beta-actin primer i always used. Dimers ...